diff --git a/tests/layer_tests/pytorch_tests/test_sub.py b/tests/layer_tests/pytorch_tests/test_sub.py
new file mode 100644
index 00000000000..95d3d0dc763
--- /dev/null
+++ b/tests/layer_tests/pytorch_tests/test_sub.py
@@ -0,0 +1,36 @@
+# Copyright (C) 2018-2023 Intel Corporation
+# SPDX-License-Identifier: Apache-2.0
+
+import numpy as np
+import pytest
+import torch
+
+from pytorch_layer_test_class import PytorchLayerTest
+
+
+class TestSub(PytorchLayerTest):
+    def _prepare_input(self):
+        return self.input_data
+
+    def create_model(self):
+
+        class aten_sub(torch.nn.Module):
+
+            def forward(self, x, y, alpha: float):
+                return torch.sub(x, y, alpha=alpha)
+
+        ref_net = None
+
+        return aten_sub(), ref_net, "aten::sub"
+
+    @pytest.mark.parametrize('input_data', [(np.random.randn(2, 3, 4).astype(np.float32),
+                                             np.random.randn(2, 3, 4).astype(np.float32),
+                                             np.random.randn(1)),
+                                            (np.random.randn(4, 2, 3).astype(np.float32),
+                                             np.random.randn(1, 2, 3).astype(np.float32),
+                                             np.random.randn(1)),])
+    @pytest.mark.nightly
+    @pytest.mark.precommit
+    def test_sub(self, ie_device, precision, ir_version, input_data):
+        self.input_data = input_data
+        self._test(*self.create_model(), ie_device, precision, ir_version)