diff --git a/tools/interop_matrix/client_matrix.py b/tools/interop_matrix/client_matrix.py
index ad49f41e35e..c400c5971ac 100644
--- a/tools/interop_matrix/client_matrix.py
+++ b/tools/interop_matrix/client_matrix.py
@@ -124,6 +124,7 @@ LANG_RELEASE_MATRIX = {
             ('v1.49.1', ReleaseInfo()),
             ('v1.52.0', ReleaseInfo()),
             ('v1.53.0', ReleaseInfo()),
+            ('v1.54.0', ReleaseInfo()),
         ]),
     'go':
         OrderedDict([
@@ -449,6 +450,9 @@ LANG_RELEASE_MATRIX = {
                 ('v1.53.0',
                  ReleaseInfo(runtimes=['python'],
                              testcases_file='python__master')),
+                ('v1.54.0',
+                 ReleaseInfo(runtimes=['python'],
+                             testcases_file='python__master')),
             ]),
     'node':
         OrderedDict([
@@ -527,6 +531,7 @@ LANG_RELEASE_MATRIX = {
             ('v1.49.1', ReleaseInfo()),
             ('v1.52.0', ReleaseInfo()),
             ('v1.53.0', ReleaseInfo()),
+            ('v1.54.0', ReleaseInfo()),
         ]),
     'php':
         OrderedDict([
@@ -578,6 +583,7 @@ LANG_RELEASE_MATRIX = {
             ('v1.49.1', ReleaseInfo()),
             ('v1.52.0', ReleaseInfo()),
             ('v1.53.0', ReleaseInfo()),
+            ('v1.54.0', ReleaseInfo()),
         ]),
     'csharp':
         OrderedDict([